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A collection of readily reproducible methods for the design, preparation, and use of RNAs for silencing gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of si RNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of micro RNAs. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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Ngôn ngữ Anh ● định dạng EPUB ● ISBN 9781592599356 ● Biên tập viên Gordon Carmichael ● Nhà xuất bản Humana Press ● Được phát hành 2008 ● Có thể tải xuống 3 lần ● Tiền tệ EUR ● TÔI 8839620 ● Sao chép bảo vệ Adobe DRM
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